Bio - chemical biomarkers in algae Scenedesmus obliquus exposed to heavy metals Cd , Cu and Zn Yahia
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چکیده
Laboratory studies were conducted to determine the effects of different concentrations of Cu, Cd, Zn and mixture (equal concentrations from the three heavy metals) on growth and some oxidative stress (catalase and glutathione reductase) on Scenedesmus obliquus (microalgae) after exposure for 24, 48, and 96 h. In addition, the uptake of Cu, Cd and Zn were determined in the culture medium after 24, 48 and 96h of exposure. The values of LC10 were (99.4 ± 3.8, 120.3 ± 3.4 and 75.6 ± 3.9 μg.L −1 after 24 h for Cu, Cd and Zn, respectively). The catalase (CAT) and glutathione reductase (GR) enzyme activities were used as biomarkers to evaluate the toxic effects of Cu, Cd, Zn and in the mixture on the microalgae. Enzymatic activities were measured in the presence of each compound alone after 24, 48 and 96 h and also in mixture after the same time of exposure. While CAT activity which increase by low concentrations, started to decrease with the higher concentrations (50 -100 μg.L). The tested heavy metals (Cu, Cd and Zn) show a significant increase in up take within concentrations. The results showed that Cu, Cd, Zn and mixture induced antioxidative enzyme activities (CAT and GR) at different concentrations. Additionally, a decrease in Chl.a, Chl.b and caritenoids was observed in algae after exposure to Cu, Cd, Zn and mixture. [Yahia Y. I. Mosleh and Jelan Mofeed. Bio-chemical biomarkers in algae Scenedesmus obliquus exposed to heavy metals Cd, Cu and Zn. Life Sci J 2014;11(10):994-1004]. (ISSN:1097-8135). http://www.lifesciencesite.com. 153
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